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MPromDb (Mammalian Promoter Database) is a curated database of gene promoters identified from ChIP-seq The proximal promoter region (upstream of the core-promoter region) contains the cis-regulatory elements of most of the transcription factors (TFs).〔Hao Sun, Saranyan K. Palaniswamy, Twyla T. Pohar. MPromDb: an integrated resource for annotation and visualization of mammalian gene promoters and ChIP-chip experimental data. ''Nucleic Acids Research'', 2006, 34: 98-103. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1347458/〕 Recently a better approach to annotate active promoters has been demonstrated with a combination of ChIP-seq and computational technique.〔Sun,H., Wu,J., Wickramasinghe,P., Pal,S., Gupta,R., Bhattacharya,A., Agosto-Pe´ rez,F.J., Showe,L.C., Huang,T.H. and Davuluri,R.V. (2010) Genome-wide mapping of RNA Pol-II promoter usage in mouse tissues by ChIP-seq. Nucleic Acids Res., PMID 20843783. http://nar.oxfordjournals.org/content/early/2010/09/14/nar.gkq775.full.pdf+html〕 This technique has been used to find the target genes of TFs in mammalian systems.〔Weinmann,A.S., Yan,P.S., Oberley,M.J., Huang,T.H. and Farnham,P.J. (2002) Isolating human transcription factor targets by coupling chromatin immunoprecipitation and CpG island microarray analysis. ''Genes Dev''., 16, 235–244. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC155318/ 〕 The MPromDb is based on this technology. Curated promoter sequences for eukaryotic organisms are provided by EPD database,〔Schmid,C.D., Perier,R., Praz,V. and Bucher,P. EPD in its twentieth year: towards complete promoter coverage of selected model organisms. ''Nucleic Acids Res'', 2006, 34: 82–85. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1347508/ 〕 however, promoter activity information at tissue/ cell centric level is not offered. The MPromDb data base added active RNAP-II promoters identified after analyzing ten different mouse cell/tissue ChIP-seq experiments performed with RNAP-II antibody and six different human cell types.〔 The data was acquired by a series of computational methods followed by manual correction to ensure its high level quality.〔 In the newest version of MPromDb, about 507 million uniquely aligned RNA Pol-II ChIP-seq reads have already been analyzed from 26 different databases, including six human cell-types and 10 distinct mouse cell/tissues.〔 ==References== 〔 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Mammalian promoter database」の詳細全文を読む スポンサード リンク
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